H2kb specific T cells
Functional CD8 T cells have been detected in vivo after SV40 Tag specific-vaccination of bioluminescent S40 Large T expression in mice (Buschow et al.) This model can be used to compare in vivo levels of interferon gamma in mice to demonstrate the sensitivity of in vivo bioluminescent imaging. Since the described transgenic Lox-Tag Luc mice contain also the Chloramphinicol-Transferase (CAT) gene within the stop cassette, it could be also possible to analyse gene expression using CAT assays.
Resources
- Buschow C, Charo J, Anders K, Loddenkemper C, Jukica A, Alsamah W, Perez C, Willimsky G, Blankenstein T, 2010 Mar 15. "In vivo imaging of an inducible oncogenic tumor antigen visualizes tumor progression and predicts CTL tolerance." J Immunol 184 (6): 2930-8 [PubMed]
Last modified: 27.03.2025 - 12:05:51
